Jumat, 18 Juni 2010

Teknik Biomedis

BENG 100: Frontiers of Biomedical Engineering

Lecture 4 - Genetic Engineering (cont.)


Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter.

Reading assignment:

Biomedical Engineering: Bridging Medicine and Technology, in preparation by Mark Saltzman (forthcoming by Cambridge University Press); chapter 3
Summary and Key Concepts: Chapter 3 [PDF]

Class lecture:

medium bandwidth
low bandwidth
high bandwidth


Problem Set 2 [PDF]

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